Animal Tissue

MOUSE AND SAHARA GERBIL LIVER

Sample preparation according to the following protocol: :

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.
levure-3 (1)
Hepatocyte – perinuclear region
levure2 (1)
levure1
Overview of a Sahara gerbil hepatocyte.

Neuromuscular

Muscle – Mouse nerve

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – 1 minute UranyLess contrast – 1 minute Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

Intestine

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

Kidney

Mouse Kidney

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – 1 minute UranyLess contrast – 1 minute Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

Delta-Rein3-1024x1010
Mouse kidney
rein2-933x1024
Basal invaginations – hemi- desmosomes – basal blade: increase the exchange surface – Kidney
Delta-rein4-1024x1014
Glomerular Zone – podocytes – stalks – Kidney.
Delta-rein31-1024x946
Podocytes – stalks – Kidney.

Andrenal Gland

Adrenal gland Sahara gerbil

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

cortico-surrénale-6
Glomerular Zone – podocytes – stalks – Kidney.

Adrenal gland Sahara gerbil

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

Delta-Rein3-1024x1010
Mouse kidney
rein2-933x1024
Basal invaginations – hemi- desmosomes – basal blade: increase the exchange surface – Kidney

Ovarian - Ovarian Follicle - Uranyless

Mouse ovarian follicle

We have tested UranyLess on different accessories cells constituting the mouse ovarian follicle.

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

theque-interne
Internal theca of mouse ovarian follicle.
theque-interne-2
Mitochondria fingers typical glove steroid synthesis in cells (Internal theca of mouse ovarian follicle).
theque-externe1 (1)
External theca cell of a mouse ovarian follicle.
Delta-cellule-folliculaire
Follicular cell of the mouse ovarian follicle Corona Radiata.

Cardiac muscle - Uranyless

Mouse cardiac muscle

Sample preparation according to the following protocol: :

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.
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